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Angew Chem Int Ed Engl. 2014 Jul 28;53(31):8099-103. doi: 10.1002/anie.201403812. Epub 2014 Jun 24.

Optical-resolution photoacoustic microscopy for volumetric and spectral analysis of histological and immunochemical samples.

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  • 1The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332 (USA).

Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) is an imaging modality with superb penetration depth and excellent absorption contrast. Here we demonstrate, for the first time, that this technique can advance quantitative analysis of conventional chromogenic histochemistry. Because OR-PAM can quantify the absorption contrast at different wavelengths, it is feasible to spectrally resolve the specific biomolecules involved in a staining color. Furthermore, the tomographic capability of OR-PAM allows for noninvasive volumetric imaging of a thick sample without microtoming it. By immunostaining the sample with different chromogenic agents, we further demonstrated the ability of OR-PAM to resolve different types of cells in a coculture sample with imaging depths up to 1 mm. Taken together, the integration of OR-PAM with (immuno)histochemistry offers a simple and versatile technique with broad applications in cell biology, pathology, tissue engineering, and related biomedical studies.

© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

KEYWORDS:

biomedical imaging; cell biology; histochemistry; photoacoustic microscopy; tissue engineering

PMID:
24961608
[PubMed - in process]
PMCID:
PMC4116434
[Available on 2015-07-28]
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