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C R Biol. 2014 Jun;337(6):383-90. doi: 10.1016/j.crvi.2014.03.005. Epub 2014 May 10.

Factors influencing in vitro shoot regeneration from leaf segments of Chrysanthemum.

Author information

  • 1Department of Horticultural Science, Kyungpook National University, Daegu 702-701, Republic of Korea. Electronic address: naingaunghtay2008@gmail.com.
  • 2Department of Horticultural Science, Kyungpook National University, Daegu 702-701, Republic of Korea.
  • 3Department of Ecological Environment, Kyungpook National University, Sangju 742-711, Republic of Korea.
  • 4National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
  • 5Department of Horticultural Science, Kyungpook National University, Daegu 702-701, Republic of Korea. Electronic address: ckkim@knu.ac.kr.

Abstract

The objective of this research was to develop an efficient protocol for shoot regeneration from leaf segments of the Chrysanthemum cv. Vivid Scarlet by examining the effects of plant growth regulators, dark incubation period, gelling agents, and silver nitrate. The highest number of shoots per explant (12.3) was regenerated from leaf explants cultured on Murashige and Skoog (MS) medium supplemented with a combination of 1 mgL(-1) of 6-benzyladenine (BA) and 2 mgL(-1) of α-naphthaleneacetic acid (NAA) under light conditions without any initial dark period. Gelrite was the most effective gelling agent for shoot regeneration among those tested, whereas the presence of silver nitrate distinctly inhibited shoot regeneration. Superior plant growth and rooting was observed on a hormone-free MS medium solidified with Gelrite. Flow cytometry analysis revealed no ploidy variation between the regenerated plants and the mother plant grown under greenhouse conditions. The established protocol was applicable to shoot regeneration for four out of six cultivars tested. This research will facilitate the genetic transformation and micropropagation of Chrysanthemum cultivars.

Copyright © 2014. Published by Elsevier SAS.

KEYWORDS:

Flow cytometry; Gelling agent; Ornamental plant; Plant growth regulator; Silver nitrate

PMID:
24961558
[PubMed - indexed for MEDLINE]
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