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Chem Commun (Camb). 2014 Aug 11;50(62):8571-4. doi: 10.1039/c4cc03949a.

One-pot synthesis of fluorescent BSA-Ce/Au nanoclusters as ratiometric pH probes.

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  • 1Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.


A facile, one-pot synthetic approach has been developed for the preparation of BSA-Ce/Au NCs. The fluorescence intensities of BSA-Ce/Au NCs at 410 and 650 nm are pH dependent and independent, respectively. The fluorescence intensity ratio (I410/I650) is linear against pH values from 6.0 to 9.0. These stable and biocompatible BSA-Ce/Au NCs have been used as ratiometric probes for monitoring local pH values inside HeLa cells.

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