Molecular cloning, characterization and expression of miR-15a-3p and miR-15b-3p in dairy cattle

Zhixiong Li; Huilin Zhang; Ning Song; Hongliang Wang; Ling Chen; Mengxing Zhai; Xiaolin Liu

doi:10.1016/j.mcp.2014.06.003

Molecular cloning, characterization and expression of miR-15a-3p and miR-15b-3p in dairy cattle

Mol Cell Probes. 2014 Oct-Dec;28(5-6):255-8. doi: 10.1016/j.mcp.2014.06.003. Epub 2014 Jun 19.

Authors

Zhixiong Li¹, Huilin Zhang¹, Ning Song², Hongliang Wang¹, Ling Chen¹, Mengxing Zhai¹, Xiaolin Liu³

Affiliations

¹ College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China.
² PLA Air Force Xi'an Flight Academy, Huxian, Xi'an, Shaanxi 710306, China.
³ College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China. Electronic address: liuxiaolin@nwsuaf.edu.cn.

PMID: 24951895
DOI: 10.1016/j.mcp.2014.06.003

Abstract

MicroRNA (miRNA) plays an important role in modulating immune responses. We identified miR-15a-3p and miR-15b-3p which were immune related in bovine. Fourteen potential target genes were predicted. Both miR-15a-3p and miR-15b-3p showed significant differential expression patterns in healthy and mastitis infected cattle (P < 0.01). Expression patterns of the two miRNAs provide important information on the relationships between mastitis and miRNA expression.

Keywords: Dairy cattle; Mastitis; Stem-loop qPCR; miR-15a-3p; miR-15b-3p.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Cattle
Cloning, Molecular
Female
Gene Expression Profiling*
Mammary Glands, Animal / metabolism*
Mammary Glands, Animal / pathology
Mastitis, Bovine / genetics*
MicroRNAs / genetics*
Molecular Sequence Data
Reverse Transcriptase Polymerase Chain Reaction

Substances

MicroRNAs