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World J Gastroenterol. 2014 Jun 14;20(22):6884-96. doi: 10.3748/wjg.v20.i22.6884.

Mesothelin promotes cell proliferation in the remodeling of neonatal rat pancreas.

Author information

  • Dan-Dan Yin, Department of Central Laboratory, the Second Affiliated Hospital of Southeast University, Nanjing 210003, Jiangsu Province, China.

Abstract

AIM:

To investigate the effect of mesothelin in the remodeling of the endocrine pancreas in neonatal rats.

METHODS:

Overexpression or downregulation of mesothelin expression in INS-1 cells was carried out to investigate the effect of mesothelin during cell proliferation and cell apoptosis in vitro. Adenovirus-mediated RNA interference was performed to block mesothelin in vivo to directly assess the role of mesothelin in the remodeling of the endocrine pancreas in neonatal rats.

RESULTS:

Exogenous overexpression of mesothelin promoted cell proliferation, cell colony formation and enhanced cell resistance to apoptosis of INS-1 cells. Down-regulation of mesothelin made no difference in cell proliferation and apoptosis compared with that in the control group. After an injection of adenovirus-mesothelin, a significantly increased number of small islets appeared, and the expression of PCNA was decreased on day 7 and day 14 compared with the Ad-EGFP group.

CONCLUSION:

Mesothelin was able to promote β cell proliferation in the remodeling stage of neonatal rats. Mesothelin may have an important role in the remodeling of the endocrine pancreas in neonatal rats.

KEYWORDS:

Cell proliferation; Mesothelin; Pancreatic development; Pancreatic remodeling; Small islets

PMID:
24944479
[PubMed - in process]
PMCID:
PMC4051928
Free PMC Article

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