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Biotechnol Bioeng. 2014 Dec;111(12):2486-98. doi: 10.1002/bit.25313. Epub 2014 Sep 2.

Scale-down characterization of post-centrifuge flocculation processes for high-throughput process development.

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  • 1The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK; Lonza Biologics plc, Slough, Berkshire, SL1 4DX, UK.


The authors present a novel ultra scale-down (USD) methodology for the characterization of flocculation processes. This USD method, consisting of a multiwell, magnetically agitated system that can be fitted on the deck of a liquid handling robot, mimicked the flocculation performance of a nongeometrically similar pilot-scale vessel representing greater than three orders of magnitude scale-up. Mixing scales (i.e. macromixing, mesomixing or micromixing) modulated the flocs' size and determined the success of some of the scale-up correlations reviewed in the literature.

© 2014 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals, Inc.


flocculation; high-throughput; micromixing; primary separation; scale-up; ultra scale-down

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