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Genetics. 2014 Aug;197(4):1165-74. doi: 10.1534/genetics.114.167007. Epub 2014 Jun 14.

A critical component of meiotic drive in neurospora is located near a chromosome rearrangement.

Author information

  • 1School of Biological Sciences, Illinois State University, Normal, Illinois 61790.
  • 2Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211.
  • 3School of Biological Sciences, Illinois State University, Normal, Illinois 61790 tmhammo@ilstu.edu.

Abstract

Neurospora fungi harbor a group of meiotic drive elements known as Spore killers (Sk). Spore killer-2 (Sk-2) and Spore killer-3 (Sk-3) are two Sk elements that map to a region of suppressed recombination. Although this recombination block is limited to crosses between Sk and Sk-sensitive (Sk(S)) strains, its existence has hindered Sk characterization. Here we report the circumvention of this obstacle by combining a classical genetic screen with next-generation sequencing technology and three-point crossing assays. This approach has allowed us to identify a novel locus called rfk-1, mutation of which disrupts spore killing by Sk-2. We have mapped rfk-1 to a 45-kb region near the right border of the Sk-2 element, a location that also harbors an 11-kb insertion (Sk-2(INS1)) and part of a >220-kb inversion (Sk-2(INV1)). These are the first two chromosome rearrangements to be formally identified in a Neurospora Sk element, providing evidence that they are at least partially responsible for Sk-based recombination suppression. Additionally, the proximity of these chromosome rearrangements to rfk-1 (a critical component of the spore-killing mechanism) suggests that they have played a key role in the evolution of meiotic drive in Neurospora.

Copyright © 2014 by the Genetics Society of America.

KEYWORDS:

Spore killer; genome sequencing and mapping; meiosis; meiotic drive; selfish elements

PMID:
24931406
[PubMed - in process]
PMCID:
PMC4125391
[Available on 2015/8/1]
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