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Nat Mater. 2014 Aug;13(8):829-36. doi: 10.1038/nmat3998. Epub 2014 Jun 15.

Gradated assembly of multiple proteins into supramolecular nanomaterials.

Author information

  • 11] Department of Surgery, University of Chicago, 5841 S. Maryland Avenue ML 5032 Chicago, Illinois 60637, USA [2].
  • 2Department of Surgery, University of Chicago, 5841 S. Maryland Avenue ML 5032 Chicago, Illinois 60637, USA.
  • 31] Department of Surgery, University of Chicago, 5841 S. Maryland Avenue ML 5032 Chicago, Illinois 60637, USA [2] Illinois Institute of Technology, Department of Biomedical Engineering, 3300 S. Federal Street Chicago, Illinois 60616, USA.
  • 41] Department of Surgery, University of Chicago, 5841 S. Maryland Avenue ML 5032 Chicago, Illinois 60637, USA [2] Committee on Immunology, University of Chicago, 5841 S. Maryland Avenue ML 5032 Chicago, Illinois 60637, USA.
  • 51] Department of Surgery, University of Chicago, 5841 S. Maryland Avenue ML 5032 Chicago, Illinois 60637, USA [2] Committee on Immunology, University of Chicago, 5841 S. Maryland Avenue ML 5032 Chicago, Illinois 60637, USA [3] Committee on Molecular Medicine, University of Chicago, 5841 S. Maryland Avenue ML 5032 Chicago, Illinois 60637, USA.

Abstract

Biomaterials exhibiting precise ratios of different bioactive protein components are critical for applications ranging from vaccines to regenerative medicine, but their design is often hindered by limited choices and cross-reactivity of protein conjugation chemistries. Here, we describe a strategy for inducing multiple different expressed proteins of choice to assemble into nanofibres and gels with exceptional compositional control. The strategy employs 'βTail' tags, which allow for good protein expression in bacteriological cultures, yet can be induced to co-assemble into nanomaterials when mixed with additional β-sheet fibrillizing peptides. Multiple different βTail fusion proteins could be inserted into peptide nanofibres alone or in combination at predictable, smoothly gradated concentrations, providing a simple yet versatile route to install precise combinations of proteins into nanomaterials. The technology is illustrated by achieving precisely targeted hues using mixtures of fluorescent proteins, by creating nanofibres bearing enzymatic activity, and by adjusting antigenic dominance in vaccines.

PMID:
24930032
[PubMed - indexed for MEDLINE]
PMCID:
PMC4180598
Free PMC Article
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