Format

Send to

Choose Destination
See comment in PubMed Commons below
J Photochem Photobiol B. 2014 Sep 5;138:109-17. doi: 10.1016/j.jphotobiol.2014.05.011. Epub 2014 May 27.

Binding properties of herbicide chlorpropham to DNA: spectroscopic, chemometrics and modeling investigations.

Author information

  • 1State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi, China.
  • 2State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi, China. Electronic address: gwzhang@ncu.edu.cn.

Abstract

The binding properties of chlorpropham (CIPC) to calf thymus DNA (ctDNA) were investigated in vitro by UV-vis absorption, fluorescence, circular dichroism (CD) and Fourier transform infrared (FT-IR) spectroscopy coupled with molecular modeling method. The results obtained from UV-vis absorption, fluorescence and CD spectroscopic methods as well as DNA viscosity and melting measurements indicated that the binding of CIPC to ctDNA was an intercalative mode. The FT-IR analysis and molecular modeling showed that CIPC mainly bound to guanine base of ctDNA. The association constant of the ctDNA-CIPC complex was determined to be in the order of 10(4) L mol(-1) by fluorescence titration. The calculated enthalpy change and entropy change suggested that hydrophobic forces and hydrogen bonds played prominent roles in the binding process. Furthermore, multivariate curve resolution-alternating least squares (MCR-ALS) approach was used to analyze the combined UV-vis absorption data matrix from the CIPC-ctDNA reaction system. The concentration profiles of the reaction components (CIPC, ctDNA and CIPC-ctDNA complex) and their pure spectra were successfully obtained to monitor the process of CIPC interaction with ctDNA. This study may contribute to the understanding of the CIPC-ctDNA interaction mechanism and toxicological effect of CIPC at the molecular level.

Copyright © 2014 Elsevier B.V. All rights reserved.

KEYWORDS:

Binding properties; Calf thymus DNA; Chlorpropham; Intercalation; Multivariate curve resolution-alternating leas squares

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk