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Exp Ther Med. 2014 Jun;7(6):1738-1744. Epub 2014 Mar 10.

Effects of methylthiouracil on the proliferation and apoptosis of rat bone marrow stromal cells.

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  • 1Department of Pediatrics, Affiliated Hospital and First Clinical College, Guangdong Medical College, Zhanjiang, Guangdong 524001, P.R. China.

Abstract

The aim of the present study was to investigate the effects of methylthiouracil (MTU) on the proliferation and apoptosis of rat bone marrow stromal cells (BMSCs). Rat BMSCs were isolated, cultured in vitro and treated with various concentrations of MTU. Cell growth curves were determined using the Cell Counting Kit-8 method and the effect of MTU on BMSCs in a logarithmic growth phase was observed. BMSC apoptosis following MTU treatment was detected by flow cytometry. The experimental results demonstrated that the proliferation-inhibition effect was gradually enhanced with increasing MTU concentrations and the extension of treatment time. Statistically significant differences were observed between the treatment and the control groups (P<0.05). In addition, the BMSC apoptosis rate gradually increased with increasing drug concentrations and treatment time extension; statistically significant differences were observed between the treatment and the control groups (P<0.05). Therefore, the results of the present study demonstrated that MTU inhibited the proliferation of BMSCs and promoted apoptosis, indicating the cytotoxic effects of MTU on BMSCs.

KEYWORDS:

apoptosis; bone marrow stromal cells; methylthiouracil; proliferation

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