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Luminescence. 2015 May;30(3):263-8. doi: 10.1002/bio.2723. Epub 2014 Jun 13.

Determination of selenium via the fluorescence quenching effect of selenium on hemoglobin-catalyzed peroxidative reaction.

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  • 1Department of Chemistry, Zhoukou Normal University, Zhoukou, 466001, China; Department of Chemistry, Zhengzhou University, Zhengzhou, 450001, China.


A new method for the determination of selenium based on its fluorescence quenching on the hemoglobin-catalyzed reaction of H2 O2 and l-tyrosine has been established. The effect of pH, foreign ions and the optimization of variables on the determination of selenium was examined. The calibration curve was found to be linear between the fluorescence quenching (F0 /F) and the concentration of selenium within the range of 0.16-4.00 µg/mL. The detection limit was 1.96 ng/mL and the relative standard deviation was 3.14%. This method can be used for the determination of selenium in Se-enriched garlic bulbs with satisfactory results.

Copyright © 2014 John Wiley & Sons, Ltd.


enzyme-catalyzed; fluorescence quenching; selenium

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