Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Pharmacogn Mag. 2014 Apr;10(38):165-71. doi: 10.4103/0973-1296.131030.

Effect of ambrex (a herbal formulation) on oxidative stress in hyperlipidemic rats and differentiation of 3T3-L1 preadipocytes.

Author information

  • 1Department of Biotechnology, Rajalakshmi Engineering College (Affiliated to Anna University), Thandalam, Chennai, Tamil Nadu, India.

Abstract

BACKGROUND:

Ambrex is a polyherbal formulation which consists of Withania somnifera, Orchis mascula, Cycas circirnalis, Shorea robusta with amber.

OBJECTIVE:

The present study was designed to explore the potential effects of ambrex on the antioxidant status in high fat diet fed rats and to investigate the possible mechanisms focusing on the gene expression involved in adipogenesis and inflammation in 3T3-L1 cell line.

MATERIALS AND METHODS:

Male Wistar rats were divided into four groups (n = 6); Group A received normal diet, Group B received high fat diet for 30 days, Group C and D received high fat diet for 30 days and treated with ambrex (40 mg/kg b.w) and atorvastatin (10 mg/kg b.w) for successive 15 days respectively. This study also assesses the effect of ambrex on adipogenesis in 3T3-L1 adipocytes.

RESULTS:

The serum total cholesterol and triglycerides were significantly decreased in ambrex treated hyperlipidemic animals when compared to untreated animals. The activities of catalase, superoxide dismutase and reduced glutathione were significantly augmented in the serum, liver, and heart of hyperlipidemic rats treated with ambrex when compared to control. Ambrex treated rats had significant reductions in malondiadehyde levels in the serum, liver and heart compared to untreated rats. In addition, we observed that treatment with ambrex resulted in a major inhibition of pre-adipocyte differentiation of 3T3-L1 cells in vitro by suppression of peroxisome proliferator activated receptor gamma, sterol regulatory binding proteins, tumor necrosis factor-α, inducible nitricoxide synthase, leptin, and upregulation of thioredoxin 1 (TRX1) and TRX2 mRNA expression.

CONCLUSION:

Therefore, ambrex may be a potential drug for treatment of hyperlipidemia and related disorders.

KEYWORDS:

3T3-L1 cell line; antioxidants; oxidative stress; peroxisome proliferator activated receptor gamma; sterol regulatory binding proteins

PMID:
24914283
[PubMed]
PMCID:
PMC4048564
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Medknow Publications and Media Pvt Ltd Icon for PubMed Central
    Loading ...
    Write to the Help Desk