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Int J Gynaecol Obstet. 2014 Sep;126(3):256-9. doi: 10.1016/j.ijgo.2014.03.035. Epub 2014 May 22.

Placental proteome alterations in women with intrahepatic cholestasis of pregnancy.

Author information

  • 1First People's Hospital of Yuhang District, Hangzhou, China.
  • 2Ningbo Institute of Medical Sciences, Medical School, Ningbo University, Ningbo, China; Institute of Pharmacology, Toxicology and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
  • 3Institute of Pharmacology, Toxicology and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
  • 4Institute of Pharmacology, Toxicology and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China. Electronic address: chenshuqing@zju.edu.cn.

Abstract

OBJECTIVE:

To investigate differences in the placental proteomes of women with intrahepatic cholestasis of pregnancy (ICP) and those with a normal pregnancy.

METHODS:

Ten pregnant women diagnosed with ICP were recruited at the First People's Hospital of Yuhang District from October 2011 to September 2012; 10 age-matched healthy pregnant women acted as controls. Total placental proteins were extracted and subjected to two-dimensional polyacrylamide gel electrophoresis followed by mass spectrometry to identify proteins that were differentially expressed in the two groups.

RESULTS:

In total, 37 protein spots with differentially expressed proteins were found. These comprised proteins involved in cytoskeleton activity, blood coagulation, and platelet activation as well as chaperones, heat shock proteins, RNA-binding and calcium-binding proteins, and various enzymes.

CONCLUSION:

The placentas of women with ICP displayed significant proteome differences compared with women with a normal pregnancy. The results indicate that a variety of mechanisms and proteins may contribute to the development of ICP. Further verification and research are required to elucidate the exact roles of these proteins in ICP pathogenesis.

Copyright © 2014 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.

KEYWORDS:

2D-PAGE; Intrahepatic cholestasis of pregnancy; Mass spectrometry; Placenta; Proteomics

PMID:
24913202
[PubMed - in process]
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