Use of COI, CytB and ND5 genes for intra- and inter-specific differentiation of Haematobia irritans and Haematobia exigua

Van Lun Low; Tiong Kai Tan; Phaik Eem Lim; Luísa Nogueira Domingues; Sun Tee Tay; Yvonne Ai Lian Lim; Thary Gazi Goh; Chandrawathani Panchadcharam; Premaalatha Bathmanaban; Mohd Sofian-Azirun

doi:10.1016/j.vetpar.2014.05.036

Use of COI, CytB and ND5 genes for intra- and inter-specific differentiation of Haematobia irritans and Haematobia exigua

Vet Parasitol. 2014 Aug 29;204(3-4):439-42. doi: 10.1016/j.vetpar.2014.05.036. Epub 2014 May 29.

Affiliations

¹ Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: lucaslow24@gmail.com.
² Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
³ Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; Institute of Ocean and Earth Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia.
⁴ Louisiana State University Agricultural Center, Department of Entomology, 404 Life Science Building, Baton Rouge, LA 70803, United States.
⁵ Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
⁶ Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
⁷ Veterinary Research Institute, 59, Jalan Sultan Azlan Shah, 31400 Ipoh, Perak, Malaysia.

PMID: 24912955
DOI: 10.1016/j.vetpar.2014.05.036

Abstract

A multilocus sequence analysis using mitochondria-encoded cytochrome c oxidase subunit I (COI), cytochrome B (CytB), NADH dehydrogenase subunit 5 (ND5); nuclear encoded 18S ribosomal RNA (18S) and 28S ribosomal RNA (28S) genes was performed to determine the levels of genetic variation between the closely related species Haematobia irritans Linnaeus and Haematobia exigua de Meijere. Among these five genes, ND5 and CytB genes were found to be more variable and informative in resolving the interspecific relationships of both species. In contrast, the COI gene was more valuable in inferring the intraspecific relationships. The ribosomal 18S and 28S sequences of H. irritans and H. exigua were highly conserved with limited intra- and inter-specific variation. Molecular evidence presented in this study demonstrated that both flies are genetically distinct and could be differentiated based on sequence analysis of mitochondrial genes.

Keywords: Buffalo fly; COI; CytB; Horn fly; Molecular markers; ND5.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Cytochromes b / genetics
Electron Transport Complex IV / genetics
Genetic Variation
Molecular Sequence Data
Multilocus Sequence Typing / veterinary
Muscidae / enzymology*
Muscidae / genetics
NADH Dehydrogenase / genetics
Sequence Analysis, DNA / veterinary

Substances

Cytochromes b
NADH Dehydrogenase
Electron Transport Complex IV

Associated data

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