Mitigated Tregs and augmented Th17 cells and cytokines are associated with severity of experimental autoimmune neuritis

Scand J Immunol. 2014 Sep;80(3):180-90. doi: 10.1111/sji.12201.

Abstract

Experimental autoimmune neuritis (EAN), an animal model of human Guillain-Barré syndrome, has long been considered as a T helper (Th) 1 cell-mediated autoimmune disorder. However, deficiency of IFN-γ, a signature Th1 cytokine, aggravated EAN, with features of elevated production of IL-17A, despite an alleviated systemic Th1 immune response. We hypothesized that Th17 cells and their cytokines might play a pathogenic role in EAN. To further clarify the roles of these Th and regulatory T cell (Treg) cytokines in the pathogenesis of EAN and their interrelationship, we investigated the expression of Th1/Th2/Th17/Treg cytokines in EAN in this study. We found that the levels of Th17 cells and IL-17A in cauda equina (CE)-infiltrating cells and splenic mononuclear cells (MNCs) as well as in serum paralleled the disease evolution, which increased progressively during the initiation stage and reached higher value at the peak of EAN. The same pattern was also noticed for the expression of IL-22. The diverse expression profiles of FoxP3, IL-17 receptors A and C were seen in CE-infiltrating cells and splenic MNCs in EAN. These findings indicate a major pro-inflammatory role of Th17 cells and IL-17A in the pathogenesis of EAN. Therapeutic interventions may be focused upon inhibiting Th17 cells and their cytokines in the early phase of EAN, so as to delay and suppress clinical signs of the disease, which has relevance for future studies on pathogenesis and treatment of GBS in humans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cauda Equina / immunology
  • Cauda Equina / metabolism
  • Cytokines / immunology*
  • Cytokines / metabolism
  • Disease Models, Animal
  • Flow Cytometry
  • Forkhead Transcription Factors / immunology
  • Forkhead Transcription Factors / metabolism
  • Guillain-Barre Syndrome / immunology
  • Guillain-Barre Syndrome / metabolism
  • Guillain-Barre Syndrome / pathology
  • Humans
  • Interferon-gamma / immunology
  • Interferon-gamma / metabolism
  • Interleukin-10 / immunology
  • Interleukin-10 / metabolism
  • Interleukin-12 / immunology
  • Interleukin-12 / metabolism
  • Interleukin-17 / blood
  • Interleukin-17 / immunology
  • Interleukin-17 / metabolism
  • Interleukin-22
  • Interleukin-6 / immunology
  • Interleukin-6 / metabolism
  • Interleukins / blood
  • Interleukins / immunology
  • Interleukins / metabolism
  • Male
  • Mice, Inbred C57BL
  • Neuritis, Autoimmune, Experimental / immunology*
  • Neuritis, Autoimmune, Experimental / metabolism
  • Neuritis, Autoimmune, Experimental / pathology
  • Receptors, Interleukin-17 / immunology
  • Receptors, Interleukin-17 / metabolism
  • Severity of Illness Index
  • Spleen / immunology
  • Spleen / metabolism
  • T-Lymphocytes, Regulatory / immunology*
  • T-Lymphocytes, Regulatory / metabolism
  • Th1 Cells / immunology
  • Th1 Cells / metabolism
  • Th17 Cells / immunology*
  • Th17 Cells / metabolism
  • Th2 Cells / immunology
  • Th2 Cells / metabolism
  • Time Factors

Substances

  • Cytokines
  • Forkhead Transcription Factors
  • Foxp3 protein, mouse
  • Interleukin-17
  • Interleukin-6
  • Interleukins
  • Receptors, Interleukin-17
  • Interleukin-10
  • Interleukin-12
  • Interferon-gamma