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Int J Tuberc Lung Dis. 2014 Jun;18(6):666-70. doi: 10.5588/ijtld.13.0848.

Same-day light-emitting diode fluorescence microscopy for the diagnosis of tuberculosis in Chhattisgarh, India.

Author information

  • 1World Health Organization Country Office for India, New Delhi, India.
  • 2International Union Against Tuberculosis and Lung Disease, South-East Asia Regional Office, New Delhi, India.
  • 3State Tuberculosis Office, Directorate of Health Services, Raipur, Chhattisgarh, India.
  • 4Intermediate Reference Laboratory, Raipur, Chhattisgarh, India.



Three medical college hospitals using light-emitting diode fluorescence microscopy (LED-FM) for diagnosing tuberculosis (TB) in Chhattisgarh, India.


To assess and compare the proportion of sputum smear-positive TB patients diagnosed through same-day microscopy (spot-spot) strategy or with the conventional (spot-morning) strategy.


During November 2012 - March 2013, all consecutively enrolled presumptive TB patients (aged ≥ 18 years) were requested to provide three specimens: two spot specimens collected 1 h apart on the first day and one early morning specimen the next day; these were stained using auramine-O and examined using LED-FM.


Of 1716 (93% of total 1845) presumptive TB patients who provided all three specimens, 218 (13%) were smear-positive: 200 (11.7%) by same-day microscopy and 217 (12.7%) by the conventional method (McNemar's χ(2) 13.5, df 1, P = 0.0002). Eighteen (8.3%) cases were missed by the same-day method.


Although LED-FM is more sensitive to paucibacillary samples, 8% of smear-positive cases were missed using the same-day method. These findings indicate the need to revisit the global applicability of the current World Health Organization recommendation of switching to same-day diagnosis from the conventional policy.

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