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Comp Biochem Physiol B Biochem Mol Biol. 2014 Aug;174:15-22. doi: 10.1016/j.cbpb.2014.05.002. Epub 2014 May 28.

Identification of fxyd genes from the spotted scat (Scatophagus argus): molecular cloning, tissue-specific expression, and response to acute hyposaline stress.

Author information

  • 1Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
  • 2Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China. Electronic address: jb-zhang@shou.edu.cn.

Abstract

By interacting with Na(+), K(+)-ATPase (NKA), the FXYD domain-containing ion transport regulator (FXYD) is involved in teleost osmoregulation, but knowledge of FXYD in marine fish is limited. In the present study, fxyd11 and fxyd12 were identified from the spotted scat (Scatophagus argus), and the two members of the FXYD protein family were expressed in a tissue-specific manner. Fxyd11 mRNA was predominantly expressed in gills, whereas fxyd12 mRNA was mainly distributed in kidneys and intestines. Acute hyposaline stress altered the activity of NKA and the expression of fxyd11 and fxyd12 in gills, kidneys, and intestines. Branchial fxyd11 mRNA expression remained at a low level during freshwater acclimation, whereas NKA activity increased, showing a negative correlation that differed from previous reports. Similarly, renal expression of fxyd11 and fxyd12 mRNA was negatively correlated with NKA activity. Unlike in gills and kidneys, intestinal NKA activity and mRNA expression of fxyd11 and fxyd12 were comparably suppressed. Taken together, the salinity-dependent expression of fxyd11 and fxyd12, and correlation with NKA activity suggested that both fxyd11 and fxyd12 were involved in the response to acute hyposaline challenge in the spotted scat.

Copyright © 2014 Elsevier Inc. All rights reserved.

KEYWORDS:

Gene expression; Osmotic stress; Scatophagus argus; fxyd

PMID:
24878493
[PubMed - in process]
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