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Kidney Int. 2014 May 28. doi: 10.1038/ki.2014.189. [Epub ahead of print]

FcγRIIB regulates T-cell autoreactivity, ANCA production, and neutrophil activation to suppress anti-myeloperoxidase glomerulonephritis.

Author information

  • 1Center for Inflammatory Diseases, Monash Health, Department of Medicine, Monash University, Melbourne, Victoria, Australia.
  • 21] Center for Inflammatory Diseases, Monash Health, Department of Medicine, Monash University, Melbourne, Victoria, Australia [2] Monash Health, Department of Nephrology, Melbourne, Victoria, Australia.
  • 31] Center for Inflammatory Diseases, Monash Health, Department of Medicine, Monash University, Melbourne, Victoria, Australia [2] Monash Health, Department of Nephrology, Melbourne, Victoria, Australia [3] Monash Health, Department of Pediatric Nephrology, Melbourne, Victoria, Australia.

Abstract

Anti-neutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis involves innate and adaptive immune cells in the induction of autoimmunity and in autoimmune effector responses. Most Fcγ receptors (FcγRs) activate immune cells, but FcγRIIB, found in humans and mice on B cells and innate cells, is an inhibitory receptor. Here we tested whether endogenous FcγRIIB negatively regulates autoreactivity and effector responses in experimental anti-myeloperoxidase (MPO) glomerulonephritis, using wild-type and FcγRIIB-/- mice. After MPO immunization, FcγRIIB-/- mice developed higher MPO-ANCA titers and increased anti-MPO T-cell responses. Transfer of FcγRIIB-deficient dendritic cells loaded with a nephritogenic MPO peptide (MPO409-428) into wild-type mice induced stronger autoimmunity than dendritic cells derived from wild-type mice. Transferring anti-MPO antibodies into lipopolysaccharide-primed mice resulted in increased glomerular neutrophil accumulation and injury in FcγRIIB-/- mice, showing a role for FcγRIIB in suppressing neutrophil activation. Inducing active autoimmunity to MPO followed by triggering T cell-mediated glomerular injury by transfer of sub-nephritogenic doses of lipopolysaccharide and anti-MPO antibodies resulted in more disease in FcγRIIB-/- mice. Thus, endogenous FcγRIIB negatively regulates anti-MPO autoimmunity and glomerulonephritis by dendritic cells, B cells, and neutrophils to limit MPO-ANCA production, T-cell responses, and neutrophil activation.Kidney International advance online publication, 28 May 2014; doi:10.1038/ki.2014.189.

PMID:
24869670
[PubMed - as supplied by publisher]
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