Non-toxic and efficient DNA extractions for soybean leaf and seed chips for high-throughput and large-scale genotyping

Zachary King; Jonathan Serrano; H Roger Boerma; Zenglu Li

doi:10.1007/s10529-014-1548-8

Non-toxic and efficient DNA extractions for soybean leaf and seed chips for high-throughput and large-scale genotyping

Biotechnol Lett. 2014 Sep;36(9):1875-9. doi: 10.1007/s10529-014-1548-8. Epub 2014 May 27.

Authors

Zachary King¹, Jonathan Serrano, H Roger Boerma, Zenglu Li

Affiliation

¹ Institute for Plant Breeding, Genetics & Genomics, The University of Georgia, Athens, GA, 30602, USA, zrking@uga.edu.

PMID: 24863292
DOI: 10.1007/s10529-014-1548-8

Abstract

In applied soybean (Glycine max L.) breeding programs, marker-assisted selection has become a necessity to select value-added quantitative trait loci. The goal of this work was to improve marker-assisted selection workflow by developing a reliable, inexpensive, high-throughput DNA extraction protocol for soybean seed and leaf samples that does not generate hazardous waste. The DNA extraction protocol developed allows for the leverage of robust SNP genotyping platforms such as the Simple Probe Assay and KASPar v4.0 SNP Genotyping System to genotype thousands of seeds or leaves non-destructively in a single day with a 95 % success rate. This methodology makes it possible to run up to 150 SNP markers on the DNA extracted from a single seed chip or leaf sample.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Plant / isolation & purification*
Genotyping Techniques / methods*
Glycine max / genetics*
High-Throughput Screening Assays / methods
Plant Leaves / genetics*
Seeds / genetics*

Substances

DNA, Plant