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J Ethnopharmacol. 2014 Aug 8;155(1):256-66. doi: 10.1016/j.jep.2014.05.021. Epub 2014 May 24.

HS-23, Lonicera japonica extract, attenuates septic injury by suppressing toll-like receptor 4 signaling.

Author information

  • 1School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
  • 2Department of New Drug Development, Huons Co., Ltd., Ansan 426-791, Republic of Korea.
  • 3Botanical Drug Research Team, Huons Co., Ltd., Ansan 426-791, Republic of Korea.
  • 4School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea. Electronic address: sunmee@skku.edu.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Lonicera japonica Thunberg is a traditional herbal medicine widely used in East Asia as an anti-bacterial, anti-inflammatory, and antiviral agent. This study was designed to investigate the effects of HS-23, ethanol extract of the dried flower buds of Lonicera japonica, in experimental models of sepsis and elucidate the mechanisms of action of HS-23.

MATERIALS AND METHODS:

Male ICR mice were intravenously administered HS-23 (20 and 40 mg/kg) for 0 (immediately) and 24 h after cecal ligation and puncture (CLP) for survival tests, and HS-23 (40 mg/kg) immediately after CLP for biochemical assays.

RESULTS:

HS-23 improved sepsis-induced mortality, enhanced bacterial clearance, and attenuated multiple organ failure. The mechanisms of action of HS-23 included attenuation of increased toll-like receptor (TLR)4 protein and mRNA expression. HS-23 suppressed sepsis-induced increases in protein expression of myeloid differentiation primary response protein 88, p38 and c-Jun N-terminal kinase in both liver and lung, as well as TIR-domain-containing adapter-inducing interferon-β and interferon regulatory transcription factor 3 protein expression in liver.

CONCLUSION:

The results of this study revealed that HS-23 attenuated sepsis through suppression of TLR signaling pathways. Therefore, our findings suggest that HS-23 might be useful as a potential therapeutic agent for treatment of sepsis.

Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

KEYWORDS:

Cecal ligation and puncture; Lonicera japonica; MyD88-dependent pathway; MyD88-independent pathway; Sepsis; Toll-like receptor

PMID:
24862492
[PubMed - indexed for MEDLINE]
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