Polymerase synthesis of photocaged DNA resistant against cleavage by restriction endonucleases

Zuzana Vaníková; Michal Hocek

doi:10.1002/anie.201402370

Polymerase synthesis of photocaged DNA resistant against cleavage by restriction endonucleases

Angew Chem Int Ed Engl. 2014 Jun 23;53(26):6734-7. doi: 10.1002/anie.201402370. Epub 2014 May 21.

Authors

Zuzana Vaníková¹, Michal Hocek

Affiliation

¹ Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, Flemingovo nám. 2, 16610 Prague 6 (Czech Republic) http://www.uochb.cas.cz/hocekgroup.

PMID: 24850380
DOI: 10.1002/anie.201402370

Abstract

5-[(2-Nitrobenzyl)oxymethyl]-2'-deoxyuridine 5'-O-triphosphate was used for polymerase (primer extension or PCR) synthesis of photocaged DNA that is resistant to the cleavage by restriction endonucleases. Photodeprotection of the caged DNA released 5-hydroxymethyluracil-modified nucleic acids, which were fully recognized and cleaved by restriction enzymes.

Keywords: DNA; DNA polymerase; nucleotides; photocaging; protective groups.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemistry*
DNA / metabolism
DNA Restriction Enzymes / metabolism*
DNA-Directed DNA Polymerase / metabolism*
Pentoxyl / analogs & derivatives*
Pentoxyl / chemistry
Pentoxyl / metabolism
Ultraviolet Rays

Substances

5-hydroxymethyluracil
Pentoxyl
DNA
DNA-Directed DNA Polymerase
DNA Restriction Enzymes