Abstract
Recently developed chemical ligation protocols were elaborated for rapid N-terminal protein PEGylation. We introduce a PEG-salicylaldehyde ester and demonstrate its site-specific ligation to the N-terminus of the RNase S protein and to the therapeutic polypeptide PTH (1-34).
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Aldehydes / chemistry*
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Humans
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Molecular Structure
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Peptide Fragments / chemistry*
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Polyethylene Glycols / chemistry*
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Protein Conformation
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Ribonucleases / chemistry*
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Serine / chemistry*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Teriparatide / analogs & derivatives*
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Teriparatide / chemistry
Substances
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Aldehydes
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Peptide Fragments
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Teriparatide
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salicylaldehyde
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Polyethylene Glycols
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Serine
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Ribonucleases
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ribonuclease S
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parathyroid hormone (1-34)amide