Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain protein

Brandon W Heimer; Tatyana A Shatova; Jungkyu K Lee; Kaja Kaastrup; Hadley D Sikes

doi:10.1039/c4an00667d

Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain protein

Analyst. 2014 Aug 7;139(15):3695-701. doi: 10.1039/c4an00667d.

Authors

Brandon W Heimer¹, Tatyana A Shatova, Jungkyu K Lee, Kaja Kaastrup, Hadley D Sikes

Affiliation

¹ Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02129, USA. sikes@mit.edu.

Abstract

Hypermethylation of CpG islands in gene promoter regions has been shown to be a predictive biomarker for certain diseases. Most current methods for methylation profiling are not well-suited for clinical analysis. Here, we report the development of an inexpensive device and an epigenotyping assay with a format conducive to multiplexed analysis.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
CpG Islands*
DNA Methylation*
DNA-Binding Proteins / analysis
DNA-Binding Proteins / metabolism*
Equipment Design
Humans
Mice
Microfluidic Analytical Techniques / instrumentation*
Nucleic Acid Hybridization
Promoter Regions, Genetic

Substances

DNA-Binding Proteins
Mbd1 protein, mouse

Abstract

Publication types

MeSH terms

Substances

Grants and funding