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Carbohydr Polym. 2014 Aug 30;109:139-47. doi: 10.1016/j.carbpol.2014.03.061. Epub 2014 Apr 1.

ZnO-modified cellulose fiber sheets for antibody immobilization.

Author information

  • 1University of West Hungary, Institute of Wood Based Products and Technologies, Bajcsy Zs. E. u. 4, 9400 Sopron, Hungary; Department of Applied Science and Engineering, Indian Institute of Technology, Roorkee, Saharanpur Campus, Saharanpur 247001, India.
  • 2University of West Hungary, Institute of Wood Based Products and Technologies, Bajcsy Zs. E. u. 4, 9400 Sopron, Hungary.
  • 3Vinča Institute of Nuclear Sciences, University of Belgrade, P.O. Box 522, 11001 Belgrade, Serbia.
  • 4Department of Bioengineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11120 Belgrade, Serbia.
  • 5Department of Applied Science and Engineering, Indian Institute of Technology, Roorkee, Saharanpur Campus, Saharanpur 247001, India.
  • 6Vinča Institute of Nuclear Sciences, University of Belgrade, P.O. Box 522, 11001 Belgrade, Serbia. Electronic address: djokovic@vinca.rs.

Abstract

Cellulose fiber sheets impregnated with saccharide capped-ZnO nanoparticles were used as bioactive materials for antibody immobilization. First, ZnO nanoparticles were synthesized in the presence of glucose (monosaccharide), sucrose (disaccharide) as well as alginic acid and starch (polysaccharides). The pine cellulose fibers were then modified by the obtained saccharide capped nanoparticles and further incorporated into the sheets. The presence of ZnO significantly improved the immobilization of the antibodies on the surface of the sheets. After rewetting the alginic acid-ZnO modified sheets with saline solution, the retention of antibodies was about 95%. A high degree of the immobilization of biomolecules is an important feature for possible fabrications of bioactive- or biosensing-papers and we successfully tested the sheets on the detection of blood types using (A, B, and D blood antibodies). The ZnO nanoparticles affected also the other properties of the sheets. The ZnO-modified fiber sheets showed higher values of tensile index (strength), smoothness and opacity, while the value of porosity was substantially lower than that of the unmodified sheet. The presence of ZnO nanoparticles provided also the antimicrobial activity to the sheets. They showed a strong activity against bacteria (Escherichia coli and Staphylococcus aureus) and strong resistance to the attack of cellulase producing fungus Gloeophyllum trabeum.

Copyright © 2014 Elsevier Ltd. All rights reserved.

KEYWORDS:

Antibody immobilization; Antimicrobial properties; Bioactive paper; Cellulose; Fibers; ZnO

[PubMed - indexed for MEDLINE]
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