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Biochem Biophys Res Commun. 2014 Jun 27;449(2):190-5. doi: 10.1016/j.bbrc.2014.04.140. Epub 2014 May 4.

miR-214 promotes the proliferation and invasion of osteosarcoma cells through direct suppression of LZTS1.

Author information

  • 1Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
  • 2Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China. Electronic address: wangtaohappy2010@sohu.com.

Abstract

Previous studies have shown that miR-214 functions either as an oncogene or a tumor suppressor in various human cancer types. The role of this microRNA in osteosarcoma (OS) is presently unclear. Here, we demonstrated that miR-214 is frequently upregulated in OS specimens, compared with noncancerous bone tissues. Bioinformatics analysis further revealed leucine zipper, putative tumor suppressor 1 (LZTS1) as a potential target of miR-214. Expression patterns of miR-214 were inversely correlated with those of LZTS1 mRNA and protein in OS tissues. Data from reporter assays showed that miR-214 directly binds to the 3'-untranslated region (3'-UTR) of LZTS1 mRNA and suppresses expression at both transcriptional and translational levels. In functional assays, miR-214 promoted OS cell proliferation, invasion and tumor growth in nude mice, which could be reversed by overexpression of LZTS1. Taken together, our data provide compelling evidence that miR-214 functions as an onco-miRNA in OS, and its oncogenic effects are mediated chiefly through downregulation of LZTS1.

Copyright © 2014 Elsevier Inc. All rights reserved.

KEYWORDS:

LZTS1; Osteosarcoma; miR-214

PMID:
24802407
[PubMed - indexed for MEDLINE]
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