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Biochem Biophys Res Commun. 2014 Jun 13;448(4):454-60. doi: 10.1016/j.bbrc.2014.04.139. Epub 2014 May 4.

Glutathione peroxidase-1 is required for self-renewal of murine embryonic stem cells.

Author information

  • 1State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis, 27 Tai-Ping Rd., Beijing 100850, China.
  • 2State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis, 27 Tai-Ping Rd., Beijing 100850, China. Electronic address: tli@ncba.ac.cn.

Abstract

Embryonic stem (ES) cells are pluripotent cells that are capable of giving rise to any type of cells in the body and possess unlimited self-renewal potential. However, the exact regulatory mechanisms that govern the self-renewal ability of ES cells remain elusive. To understand the immediate early events during ES cell differentiation, we performed a proteomics study and analyzed the proteomic difference in murine ES cells before and after a 6-h spontaneous differentiation. We found that the expression level of glutathione peroxidase-1 (GPx-1), an antioxidant enzyme, is dramatically decreased upon the differentiation. Both knockdown of GPx-1 expression with shRNA and inhibiting GPx-1 activity by inhibitor led to the differentiation of ES cells. Furthermore, we showed that during early differentiation, the quick degradation of GPx-1 was mediated by proteasome. Thus, our data indicated that GPx-1 is a key regulator of self-renewal of murine embryonic stem cells.

Copyright © 2014 Elsevier Inc. All rights reserved.

KEYWORDS:

Differentiation; Embryonic stem cells; Glutathione peroxidase-1; Self-renewal

PMID:
24802396
[PubMed - indexed for MEDLINE]
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