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Biochem Biophys Res Commun. 2014 Jun 6;448(3):267-73. doi: 10.1016/j.bbrc.2014.04.111. Epub 2014 May 2.

ω-Hydroxyundec-9-enoic acid induces apoptosis through ROS-mediated endoplasmic reticulum stress in non-small cell lung cancer cells.

Author information

  • 1Department of Biochemistry and Molecular Biology, Yonsei University College of Medicine, Seoul 120-749, Republic of Korea.
  • 2Division of Gerontology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
  • 3Department of Food Science and Engineering, Ewha Womans University, Seoul 120-750, Republic of Korea. Electronic address: jbpark06@ewha.ac.kr.

Abstract

ω-Hydroxyundec-9-enoic acid (ω-HUA), a hydroxyl unsaturated fatty acid derivative, is involved in the antifungal activity of wild rice (Oryza officinalis). Here, we investigated the anti-cancer activity of ω-HUA on a non-small cell lung cancer (NSCLC) cell line. ω-HUA increased apoptosis and induced cleavages of caspase-6, caspase-9, and poly (ADP-ribose) polymerase (PARP). ω-HUA treatment significantly induced endoplasmic reticulum (ER) stress response. Suppression of CHOP expression and inhibiting ER stress by 4-phenylbutyrate (4-PBA) significantly attenuated the ω-HUA treatment-induced activation of caspase-6, caspase-9, and PARP, and subsequent apoptotic cell death, indicating a role for ER stress in ω-HUA-induced apoptosis. In addition, cells subjected to ω-HUA exhibited significantly increased quantity of reactive oxygen species (ROS), and the ROS scavenger N-acetyl-L-cysteine (NAC) inhibited ω-HUA-induced apoptotic cell death and ER stress signals, indicating a role for ROS in ER stress-mediated apoptosis in ω-HUA-treated cells. Taken together, these results suggest that sequential ROS generation and ER stress activation are critical in ω-HUA treatment-induced apoptosis and that ω-HUA represents a promising candidate for NSCLC treatment.

Copyright © 2014 Elsevier Inc. All rights reserved.

KEYWORDS:

Apoptosis; Endoplasmic reticulum (ER) stress; N-Acetyl-l-cysteine (NAC); Non-small cell lung cancer (NSCLC); Reactive oxygen species (ROS); ω-Hydroxyundec-9-enoic acid (ω-HUA)

PMID:
24796672
[PubMed - indexed for MEDLINE]
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