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J Biosci Bioeng. 2014 Oct;118(4):441-7. doi: 10.1016/j.jbiosc.2014.04.004. Epub 2014 May 1.

Stable accumulation of seed storage proteins containing vaccine peptides in transgenic soybean seeds.

Author information

  • 1Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. Electronic address: marunobu@kais.kyoto-u.ac.jp.
  • 2Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
  • 3Hokko Chemical Industry Co., Ltd., Atsugi, Kanagawa 243-0023, Japan.
  • 4National Institute of Agrobiological Science, Tsukuba, Ibaraki 305-8602, Japan; National Agricultural Research Center for Hokkaido Region, Sapporo, Hokkaido 062-8555, Japan.
  • 5National Agricultural Research Center for Hokkaido Region, Sapporo, Hokkaido 062-8555, Japan.
  • 6Graduate School of Medicine, Hirosaki University, Hirosaki, Aomori 036-8562, Japan.

Abstract

There has been a significant increase in the use of transgenic plants for the large-scale production of pharmaceuticals and industrial proteins. Here, we report the stable accumulation of seed storage proteins containing disease vaccine peptides in transgenic soybean seeds. To synthesize vaccine peptides in soybean seeds, we used seed storage proteins as a carrier and a soybean breeding line lacking major seed storage proteins as a host. Vaccine peptides were inserted into the flexible disordered regions in the A1aB1b subunit three-dimensional structure. The A1aB1b subunit containing vaccine peptides in the disordered regions were sorted to the protein storage vacuoles where vaccine peptides are partially cleaved by proteases. In contrast, the endoplasmic reticulum (ER)-retention type of the A1aB1b subunit containing vaccine peptides accumulated in compartments that originated from the ER as an intact pro-form. These results indicate that the ER may be an organelle suitable for the stable accumulation of bioactive peptides using seed storage proteins as carriers.

Copyright © 2014 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

KEYWORDS:

Endoplasmic reticulum; Seed storage protein; Three dimensional structure; Vaccine; Vacuole

PMID:
24794626
[PubMed - indexed for MEDLINE]
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