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Eur J Obstet Gynecol Reprod Biol. 2014 Jul;178:74-9. doi: 10.1016/j.ejogrb.2014.03.049. Epub 2014 Apr 18.

Effect of 2-aminoethoxydiphenyl borate on ischemia-reperfusion injury in a rat ovary model.

Author information

  • 1Department of Obstetrics and Gynecology, Balıkesir University School of Medicine, Balikesir, Turkey. Electronic address: minetaskin1302@yahoo.com.tr.
  • 2Department of Biochemistry, Balıkesir University School of Medicine, Balikesir, Turkey.
  • 3Department of Histology and Embryology, Erciyes University School of Medicine, Kayseri, Turkey.
  • 4Department of Obstetrics and Gynecology, Balıkesir University School of Medicine, Balikesir, Turkey.
  • 5Department of Obstetrics and Gynecology, On Sekiz Mart University School of Medicine, Canakkale, Turkey.

Abstract

OBJECTIVE:

The aim of this study is to evaluate the effects of 2-aminoethoxydiphenyl borate (2-APB) as an antioxidant and analyze biochemical and histopathologic changes in experimental ischemia-reperfusion (I/R) injury in rat ovaries.

STUDY DESIGN:

Thirty female rats were utilized to create four groups. Group 1: I/R and 2-APB (2mg/kg); Group 2: I/R and 2-APB (4mg/kg); Group 3: I/R; Group 4: sham operation. Ovarian tissue and serum malondialdehyde, nitric oxide (NO) levels; ovarian tissue and serum total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) were determined. In ovarian tissue samples histopathologic examination, immunoflourescence staining by TUNEL method was studied.

RESULTS:

Tissue TOS, serum TOS, and OSI levels were elevated in I/R group. After treatment with 2-APB, tissue and serum TOS levels and OSI levels were markedly decreased. There was a significant difference in terms of tissue and serum NO levels between the sham group and I/R group. Elevation in tissue NO and serum NO levels were decreased after treatment with 2-APB. TUNEL-positive cell number gradually decreased with dose of 2-APB in groups 1 and 2.

CONCLUSION:

Conservative treatment with 2-APB is beneficial for mitigation of I/R injury, and the ovarian protective effect of 2-APB appears to be mediated through its antiapopitotic and antioxidative effects.

Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

KEYWORDS:

2-Aminoethoxydiphenyl borate (2-APB); Adnexal torsion; Ischemia-reperfusion; Rat

PMID:
24792541
[PubMed - indexed for MEDLINE]
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