Format

Send to:

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2014;1156:157-74. doi: 10.1007/978-1-4939-0685-7_10.

Determining protein subcellular localization in mammalian cell culture with biochemical fractionation and iTRAQ 8-plex quantification.

Author information

  • 1Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK, ac587@cam.ac.uk.

Abstract

Protein subcellular localization is a fundamental feature of posttranslational functional regulation. Traditional microscopy based approaches to study protein localization are typically of limited throughput, and dependent on the availability of antibodies with high specificity and sensitivity, or fluorescent fusion proteins. In this chapter we describe how Localization of Organelle Proteins by Isotope Tagging (LOPIT), a mass spectrometry based workflow coupling biochemical fractionation and iTRAQâ„¢ 8-plex quantification, can be applied for the high-throughput characterization of protein localization in a mammalian cell culture line.

PMID:
24791987
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Write to the Help Desk