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Br J Cancer. 2014 May 27;110(11):2738-46. doi: 10.1038/bjc.2014.134. Epub 2014 May 1.

Discriminating metastasised from non-metastasised seminoma based on transcriptional changes in primary tumours using NGS.

Author information

  • 11] Department of Urology, Federal Armed Forces Hospital, Hamburg 22049, Germany [2] Bundeswehr Institute of Radiobiology, Munich 80937, Germany.
  • 21] Bundeswehr Institute of Radiobiology, Munich 80937, Germany [2] Department of Urology, Federal Armed Forces Central Hospital, Koblenz 56072, Germany.
  • 31] Bundeswehr Institute of Radiobiology, Munich 80937, Germany [2] Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, MHH, Hannover, Germany.
  • 4Department of Urology, Federal Armed Forces Hospital, Hamburg 22049, Germany.
  • 5Max-Plank Institute of Psychiaty, Munich 80804, Germany.
  • 6Bundeswehr Institute of Radiobiology, Munich 80937, Germany.

Abstract

BACKGROUND:

We aimed to better discriminate (occult) metastasised from non-metastasised seminoma based on transcriptional changes of small RNAs in the primary tumour.

METHODS:

Total RNAs including small RNAs were isolated from five testicular tumours of each, lymphogenic, occult and non-metastasised patients. Next-generation sequencing (SOLID, Life Technologies) was used to examine transcriptional changes. Small RNAs showing ⩾50 reads and a significant ⩾2-fold difference using non-metastasised tumours as the reference group were examined in univariate logistic regression analysis and combinations of two small RNAs were further examined using support vector machines.

RESULTS:

On average, 1.3 × 10(7), 1.4 × 10(7) and 1.7 × 10(7) small RNA reads were detectable in non-metastasised, occult and lymphogenic metastasised seminoma, respectively, of which 30-32% remained after trimming. Between 59 and 68% represented annotated reads and between 8.6 and 11% were annotated small RNA tags. Of them, 137 small RNAs showed>50 reads and a two-fold difference to the reference. In univariate analysis, 32-38 small RNAs significantly discriminated lymphogenic/occult from non-metastasised seminoma, and among these different comparisons, it were the same small RNAs in 51-88%. Many combinations of two of these small RNAs allowed a complete discrimination of metastasised from non-metastasised seminoma irrespective of the metastasis subtype.

CONCLUSIONS:

Metastasised and non-metastasised seminoma can be completely discriminated with a combination of two small RNAs.

PMID:
24786602
[PubMed - indexed for MEDLINE]
PMCID:
PMC4037819
Free PMC Article
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