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Talanta. 2014 Jun;124:1-6. doi: 10.1016/j.talanta.2014.02.039. Epub 2014 Feb 25.

Ionic liquid-anionic surfactant based aqueous two-phase extraction for determination of antibiotics in honey by high-performance liquid chromatography.

Author information

  • 1College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
  • 2Department of Rehabilitation, the General Hospital of FAW (the Fourth Hospital of Jilin University), Haoyue Road 3788, Changchun 130011, PR China.
  • 3Department of Hand Surgery, China-Japan Union Hospital, Jilin University, Xiantai Street 126, Changchun 130033, PR China.
  • 4College of Instrumentation and Electrical Engineering, Jilin University, Qianjin Street 2699, Changchun 130012, PR China. Electronic address: vivianyxiao@163.com.

Abstract

An ionic liquid-anionic surfactant based aqueous two-phase extraction was developed and applied for the extraction of tetracycline, oxytetracycline and chloramphenicol in honey. The honey sample was mixed with Na2EDTA aqueous solution. The sodium dodecyl sulfate, ionic liquid 1-octyl-3-methylimidazolium bromide and sodium chloride were added in the mixture. After the resulting mixture was ultrasonically shaken and centrifuged, the aqueous two phase system was formed and analytes were extracted into the upper phase. The parameters affecting the extraction efficiency, such as the volume of ionic liquid, the category and amount of salts, sample pH value, extraction time and temperature were investigated. The limits of detection of tetracycline, oxytetracycline and chloramphenicol were 5.8, 8.2 and 4.2 μg kg(-1), respectively. When the present method was applied to the analysis of real honey samples, the recoveries of analytes ranged from 85.5 to 110.9% and relative standard deviations were lower than 6.9%.

Copyright © 2014 Elsevier B.V. All rights reserved.

KEYWORDS:

Anionic surfactant; Antibiotics; Aqueous two-phase extraction; Honey; Ionic liquid

PMID:
24767438
[PubMed - indexed for MEDLINE]
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