Send to:

Choose Destination
See comment in PubMed Commons below
PLoS One. 2014 Apr 23;9(4):e95398. doi: 10.1371/journal.pone.0095398. eCollection 2014.

Trypanosoma cruzi survival following cold storage: possible implications for tissue banking.

Author information

  • 1Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
  • 2University of California, Berkeley, California, United States of America.
  • 3University of Georgia, Athens, Georgia, United States of America.
  • 4Center for Medical Technology Policy, Baltimore, Maryland, United States of America.
  • 5Center for Tissue Innovation and Research, Kettering, Ohio, United States of America.
  • 6American Association of Tissue Banks, McLean, Virginia, United States of America.


While Trypanosoma cruzi, the etiologic agent of Chagas disease, is typically vector-borne, infection can also occur through solid organ transplantation or transfusion of contaminated blood products. The ability of infected human cells, tissues, and cellular and tissue-based products (HCT/Ps) to transmit T. cruzi is dependent upon T. cruzi surviving the processing and storage conditions to which HCT/Ps are subjected. In the studies reported here, T. cruzi trypomastigotes remained infective 24 hours after being spiked into blood and stored at room temperature (N = 20); in 2 of 13 parasite-infected cultures stored 28 days at 4°C; and in samples stored 365 days at -80°C without cryoprotectant (N = 28), despite decreased viability compared to cryopreserved parasites. Detection of viable parasites after multiple freeze/thaws depended upon the duration of frozen storage. The ability of T. cruzi to survive long periods of storage at +4 and -80°C suggests that T. cruzi-infected tissues stored under these conditions are potentially infectious.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk