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Nat Commun. 2014 Apr 24;5:3728. doi: 10.1038/ncomms4728.

Targeted genomic rearrangements using CRISPR/Cas technology.

Author information

  • 11] Dana-Farber Cancer Institute, Department of Medical Oncology, Boston, Massachusetts 02215, USA [2] Broad Institute of MIT and Harvard, Cancer Program, Cambridge, Massachusetts 02142, USA.
  • 21] Dana-Farber Cancer Institute, Department of Medical Oncology, Boston, Massachusetts 02215, USA [2] Broad Institute of MIT and Harvard, Cancer Program, Cambridge, Massachusetts 02142, USA [3] Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.

Abstract

Genomic rearrangements are frequently observed in cancer cells but have been difficult to generate in a highly specific manner for functional analysis. Here we report the application of CRISPR/Cas technology to successfully generate several types of chromosomal rearrangements implicated as driver events in lung cancer, including the CD74-ROS1 translocation event and the EML4-ALK and KIF5B-RET inversion events. Our results demonstrate that Cas9-induced DNA breaks promote efficient rearrangement between pairs of targeted loci, providing a highly tractable approach for the study of genomic rearrangements.

PMID:
24759083
[PubMed - indexed for MEDLINE]
PMCID:
PMC4170920
Free PMC Article
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