Format

Send to

Choose Destination
See comment in PubMed Commons below
Cytokine. 2014 Aug;68(2):137-40. doi: 10.1016/j.cyto.2014.03.011. Epub 2014 Apr 20.

Ceramide inhibits connective tissue growth factor expression by human retinal pigment epithelial cells.

Author information

  • 1Arnold and Mabel Beckman Macular Research Center, Doheny Eye Institute, Los Angeles, CA, United States.
  • 2Laboratory of Immunology, National Eye Institute, Bethesda, MD, United States.
  • 3Departments of Ophthalmology and Pathology, Keck School of Medicine USC, Los Angeles, CA, United States.
  • 4Departments of Ophthalmology and Pathology, Keck School of Medicine USC, Los Angeles, CA, United States. Electronic address: dhinton@usc.edu.

Abstract

Connective tissue growth factor (CTGF) is known to be involved in retinal fibrotic disorders. We used human retinal pigment epithelial cells (HRPE), which play critical roles in retinal fibrosis, to examine the expression of CTGF and its regulation by ceramide and TGF-β. Real-time PCR analysis showed downregulation of CTGF mRNA by C2 ceramide and upregulation by TGF-β. C2 ceramide also inhibited constitutive and TGF-β-enhanced CTGF secretion by HRPE cells. Predominant secretion (>80% of total) of CTGF from the apical side was observed in highly polarized HRPE cells. Fumonosin, an inhibitor of ceramide synthesis, stimulated CTGF secretion while 4HPR, an activator of ceramide synthesis, downregulated CTGF secretion. Based on these results demonstrating ceramide regulation of CTGF secretion by HRPE, we suggest that ceramide may have therapeutic potential for the treatment of retinal fibrotic diseases by inhibiting CTGF production.

Copyright © 2014 Elsevier Ltd. All rights reserved.

KEYWORDS:

CTGF; Ceramide; Retinal fibrosis; Retinal pigment epithelium; TGF-β

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk