Decreased level of albumin in peripheral blood mononuclear cells of streptozotocin-induced diabetic rats

J Vet Med Sci. 2014 Aug;76(8):1087-92. doi: 10.1292/jvms.13-0631. Epub 2014 Apr 23.

Abstract

We investigated the phenotypic level of albumin in peripheral blood mononuclear cells (PBMC) of streptozotocin (STZ)-induced diabetic rats. A specific reduction of albumin was identified by 2-dimensional electrophoresis and mass spectrometry. Decreased albumin content was also confirmed by immunoblotting and quantitative real-time PCR. Since albumin is a major and predominant antioxidant in plasma, the PBMC albumin may also contribute to their antioxidant activity. By measuring the amount of H2O2, lipid peroxidation and the redox form of glutathione, it was found that the production of the oxidative stress was elevated in STZ-diabetic rats compared to that of normal control. We suggest, therefore, that decreased albumin content may lead to the decreased antioxidant activity in the PBMC of type 1 diabetic rats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albumins / analysis*
  • Animals
  • DNA Primers / genetics
  • Diabetes Mellitus, Experimental / blood*
  • Diabetes Mellitus, Experimental / physiopathology
  • Electrophoresis, Gel, Two-Dimensional
  • Glutathione / metabolism
  • Hydrogen Peroxide / metabolism
  • Leukocytes, Mononuclear / chemistry*
  • Lipid Peroxidation / physiology
  • Male
  • Mass Spectrometry
  • Oxidative Stress / physiology*
  • Rats
  • Rats, Sprague-Dawley
  • Real-Time Polymerase Chain Reaction

Substances

  • Albumins
  • DNA Primers
  • Hydrogen Peroxide
  • Glutathione