Multiplexing SERS nanotags for the imaging of differentiated mouse embryonic stem cells (mESC) and detection of teratoma in vivo

Animesh Samanta; Raj Kumar Das; Sung Jin Park; Kaustabh Kumar Maiti; Young Tae Chang

Multiplexing SERS nanotags for the imaging of differentiated mouse embryonic stem cells (mESC) and detection of teratoma in vivo

Am J Nucl Med Mol Imaging. 2014 Mar 20;4(2):114-24. eCollection 2014.

Authors

Animesh Samanta¹, Raj Kumar Das², Sung Jin Park¹, Kaustabh Kumar Maiti³, Young Tae Chang⁴

Affiliations

¹ Singapore Bioimaging Consortium, Agency for Science Technology and Research (ASTAR) Singapore.
² Department of Chemistry, National University of Singapore 3 Science Drive 3, 117543, Singapore.
³ CSIR-National Institutefor Interdisciplinary Science & Technology (NIIST), Chemical Science & Technology Division (CSTD), Organic chemistry section Industrial Estate, Thiruvananthapuram - 695019, Kerala, India.
⁴ Singapore Bioimaging Consortium, Agency for Science Technology and Research (ASTAR) Singapore ; Department of Chemistry, National University of Singapore 3 Science Drive 3, 117543, Singapore.

PMID: 24753980
PMCID: PMC3992207

Abstract

Herein, we report fifty four membered, a new set of novel NIR Raman reporters and CyRLA-572 has been selected to be the best among them considering the signal intensity and stability. This new reporter molecule is an excellent partner with our in house Raman reporters (Cy7LA and Cy7.5LA). These three NIR Raman reporters are adsorbed on the gold nanoparticles to obtain their corresponding unique SERS fingerprints in which three individual characteristic peaks are capable to multiplex among them. These multiplexed Raman reporters are applied to develop biocompatible and specific targeting SERS nanotags after tagging with specific antibodies. These multiplex targeted SERS nanotags are applied to detect three targeting receptors in differentiated mouse embryonic stem cells (mESCs) consisting three germ layers such as ectoderm, mesoderm and endoderm. After successful recognition of cells by SERS techniques, we detect simultaneously three germ layers in teratoma which is a monster tumor formed from mESC cells in animal xenograft model.

Keywords: Gold nanoparticles; SERS; biocompatible; cell mapping; differentiated germ layers; ectoderm; endoderm; in vivo; mesoderm; multiplexing; nanotags; teratoma.