Identification, molecular cloning and expression analysis of five RNA-dependent RNA polymerase genes in Salvia miltiorrhiza

PLoS One. 2014 Apr 14;9(4):e95117. doi: 10.1371/journal.pone.0095117. eCollection 2014.

Abstract

RNA-dependent RNA polymerases (RDRs) act as key components of the small RNA biogenesis pathways and play significant roles in post-transcriptional gene silencing (PTGS) and antiviral defense. However, there is no information about the RDR gene family in Salvia miltiorrhiza, an emerging model medicinal plant with great economic value. Through genome-wide predication and subsequent molecular cloning, five full-length S. miltiorrhiza RDR genes, termed SmRDR1-SmRDR5, were identified. The length of SmRDR cDNAs varies between 3,262 (SmRDR5) and 4,130 bp (SmRDR3). The intron number of SmRDR genes varies from 3 (SmRDR1, SmRDR3 and SmRDR4) to 17 (SmRDR5). All of the deduced SmRDR protein sequences contain the conserved RdRp domain. Moreover, SmRDR2 and SmRDR4 have an additional RRM domain. Based on the phylogenetic tree constructed with sixteen RDRs from Arabidopsis, rice and S. miltiorrhiza, plant RDRs may be divided into four groups (RDR1-RDR4). The RDR1 group contains an AtRDR and an OsRDR, while includes two SmRDRs. On the contrary, the RDR3 group contains three AtRDRs and two OsRDRs, but has only one SmRDR. SmRDRs were differentially expressed in flowers, leaves, stems and roots of S. miltiorrhiza and responsive to methyl jasmonate treatment and cucumber mosaic virus infection. The results suggest the involvement of RDRs in S. miltiorrhiza development and response to abiotic and biotic stresses. It provides a foundation for further studying the regulation and biological functions of SmRDRs and the biogenesis pathways of small RNAs in S. miltiorrhiza.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates / pharmacology
  • Amino Acid Motifs
  • Arabidopsis / genetics
  • Base Sequence
  • Cloning, Molecular
  • Conserved Sequence
  • Cyclopentanes / pharmacology
  • Gene Expression Profiling
  • Gene Expression Regulation, Enzymologic / drug effects
  • Gene Expression Regulation, Plant* / drug effects
  • Genes, Plant*
  • Introns / genetics
  • Molecular Sequence Data
  • Organ Specificity / drug effects
  • Organ Specificity / genetics
  • Oryza / genetics
  • Oxylipins / pharmacology
  • Phylogeny
  • Protein Structure, Tertiary
  • RNA-Dependent RNA Polymerase / chemistry
  • RNA-Dependent RNA Polymerase / genetics*
  • Salvia miltiorrhiza / drug effects
  • Salvia miltiorrhiza / enzymology*
  • Salvia miltiorrhiza / genetics*

Substances

  • Acetates
  • Cyclopentanes
  • Oxylipins
  • methyl jasmonate
  • RNA-Dependent RNA Polymerase

Associated data

  • GENBANK/KF872203
  • GENBANK/KF872204
  • GENBANK/KF872205
  • GENBANK/KF872206
  • GENBANK/KF872207

Grants and funding

This work was supported by grants from the Natural Science Foundation of China (grant no. 31370327), the Beijing Natural Science Foundation (grant no. 5112026), the Major Scientific and Technological Special Project for Significant New Drugs Creation (grant no. 2012ZX09301002-001-031), the Research Fund for the Doctoral Program of Higher Education of China (grant no. 20111106110033), the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT, grant no. IRT1150), and the Program for Xiehe Scholars in Chinese Academy of Medical Sciences & Peking Union Medical College (to SL). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.