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Clin Immunol. 2014 Jul;153(1):94-103. doi: 10.1016/j.clim.2014.04.003. Epub 2014 Apr 13.

The effect of polyamines on the binding of anti-DNA antibodies from patients with SLE and normal human subjects.

Author information

  • 1Department of Rheumatology, Qilu Hospital, Shandong University, Jinan, China; Medical Research Service, Durham Veterans Administration Medical Center, Durham, NC, USA.
  • 2Medical Research Service, Durham Veterans Administration Medical Center, Durham, NC, USA; Duke University Medical Center, Durham, NC, USA.
  • 3Department of Rheumatology, Qilu Hospital, Shandong University, Jinan, China.
  • 4Medical Research Service, Durham Veterans Administration Medical Center, Durham, NC, USA; Duke University Medical Center, Durham, NC, USA. Electronic address: dpiset@acpub.duke.edu.

Abstract

Antibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus (SLE). To elucidate specificity further, the effect of polyamines on the binding of anti-DNA antibodies from patients with lupus was tested by ELISA to calf thymus (CT) DNA; we also assessed the binding of plasmas of patients and normal human subjects (NHS) to Micrococcus luteus (MC) DNA. As these studies showed, spermine can dose-dependently inhibit SLE anti-DNA binding to CT DNA and can promote dissociation of preformed immune complexes. With MC DNA as antigen, spermine failed to inhibit the NHS anti-DNA binding. Studies using plasmas adsorbed to a CT DNA cellulose affinity indicated that SLE plasmas are mixtures of anti-DNA that differ in inhibition by spermine and binding to conserved and non-conserved determinants. Together, these studies demonstrate that spermine can influence the binding of anti-DNA autoantibodies and may contribute to the antigenicity of DNA.

Published by Elsevier Inc.

KEYWORDS:

Anti-DNA antibody;; Antigenicity;; Polyamines; SLE;; Spermine;

PMID:
24732074
[PubMed - indexed for MEDLINE]
PMCID:
PMC4167072
[Available on 2015/7/1]
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