PtrKOR1 is required for secondary cell wall cellulose biosynthesis in Populus

Tree Physiol. 2014 Nov;34(11):1289-300. doi: 10.1093/treephys/tpu020. Epub 2014 Apr 11.

Abstract

KORRIGAN (KOR), encoding an endo-1,4-β-glucanase, plays a critical role in the cellulose synthesis of plant cell wall formation. KOR sequence orthologs are duplicated in the Populus genome relative to Arabidopsis. This study reports an expression analysis of the KOR genes in Populus. The five PtrKOR genes displayed different expression patterns, suggesting that they play roles in different developmental processes. Through RNAi suppression, results demonstrated that PtrKOR1 is required for secondary cell wall cellulose formation in Populus. Together, the results suggest that the PtrKOR genes may play distinct roles in association with cell wall formation in different tissues.

Keywords: KORRIGAN; cellulose synthesis; gene duplication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carbohydrate Metabolism
  • Cell Wall / metabolism
  • Cellulase / genetics*
  • Cellulase / metabolism
  • Cellulose / metabolism*
  • Gene Duplication
  • Gene Expression Regulation, Plant*
  • Molecular Sequence Data
  • Organ Specificity
  • Phylogeny
  • Plant Leaves / cytology
  • Plant Leaves / enzymology
  • Plant Leaves / genetics
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plant Stems / cytology
  • Plant Stems / enzymology
  • Plant Stems / genetics
  • Plants, Genetically Modified
  • Populus / cytology
  • Populus / enzymology*
  • Populus / genetics
  • RNA Interference
  • Sequence Alignment
  • Sequence Analysis, DNA

Substances

  • Plant Proteins
  • Cellulose
  • Cellulase