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Biomed Res Int. 2014;2014:654170. doi: 10.1155/2014/654170. Epub 2014 Feb 24.

A novel phospholipase A2 (D49) from the venom of the Crotalus oreganus abyssus (North American Grand canyon rattlesnake).

Author information

  • 1Department of Chemistry, Biochemistry and Bioprocess Engineering, Federal University of São João Del Rei, Campus Alto Paraopeba, 34420-000 Ouro Branco, MG, Brazil.
  • 2Department of Biochemistry of Biology Institute, State University of Campinas, 13083-970 Campinas, SP, Brazil.
  • 3School of Arts, Sciences and Humanities, University of São Paulo, 03828-000 São Paulo, Brazil ; Center of Natural Sciences and Humanities, Federal University of ABC, 09210-170 Santo Andre, SP, Brazil.
  • 4Center of Natural Sciences and Humanities, Federal University of ABC, 09210-170 Santo Andre, SP, Brazil.
  • 5Center of Biomolecules Study Applied to Health, Fiocruz Rondônia, Oswaldo Cruz Foundation, Medicine Department, Federal University of Rondônia, 76812-245 Porto Velho, Brazil.
  • 6Proteomics Platform, Barcelona Science Park, University of Barcelona, Baldiri Reixac, 08208 Barcelona, Spain.
  • 7Institute for Research in Biomedicine, Barcelona Science Park, University of Barcelona, 08280 Barcelona, Spain ; CIBER-BBN, Barcelona Science Park, University of Barcelona, 08280 Barcelona, Spain.
  • 8Institute for Research in Biomedicine, Barcelona Science Park, University of Barcelona, 08280 Barcelona, Spain ; CIBER-BBN, Barcelona Science Park, University of Barcelona, 08280 Barcelona, Spain ; Department of Organic Chemistry, University of Barcelona, 08028 Barcelona, Spain ; School of Chemistry and Physics, University of KwaZulu-Natal, Durban 4001, South Africa.

Abstract

Currently, Crotalus viridis was divided into two species: Crotalus viridis and Crotalus oreganus. The current classification divides "the old" Crotalus viridis into two new and independent species: Crotalus viridis (subspecies: viridis and nuntius) and Crotalus oreganus (subspecies: abyssus, lutosus, concolor, oreganus, helleri, cerberus, and caliginis). The analysis of a product from cDNA (E6d), derived from the gland of a specie Crotalus viridis viridis, was found to produce an acid phospholipase A2. In this study we isolated and characterized a PLA2 (D49) from Crotalus oreganus abyssus venom. Our studies show that the PLA2 produced from the cDNA of Crotalus viridis viridis (named E6d) is exactly the same PLA2 primary sequence of amino acids isolated from the venom of Crotalus oreganus abyssus. Thus, the PLA2 from E6d cDNA is actually the same PLA2 presented in the venom of Crotalus oreganus abyssus and does not correspond to the venom from Crotalus viridis viridis. These facts highlight the importance of performing more studies on subspecies of Crotalus oreganus and Crotalus viridis, since the old classification may have led to mixed results or mistaken data.

PMID:
24707493
[PubMed - indexed for MEDLINE]
PMCID:
PMC3953673
Free PMC Article
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