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J Virol Methods. 2014 Jul;203:107-11. doi: 10.1016/j.jviromet.2014.03.022. Epub 2014 Apr 2.

Determination of EBV serostatus prior to kidney transplantation: comparison of VIDAS®, LIAISON® and immunofluorescence assays.

Author information

  • 1Department of Laboratory Medicine (Virology), Royal Infirmary of Edinburgh, Little France, Edinburgh EH16 4SA, UK. Electronic address: i.johannessen@ed.ac.uk.
  • 2Department of Laboratory Medicine (Virology), Royal Infirmary of Edinburgh, Little France, Edinburgh EH16 4SA, UK.
  • 3Epidemiology and Statistics Core, Wellcome Trust Clinical Research Facility, Western General Hospital, Edinburgh EH4 2XU, UK.

Abstract

Immunosuppression following solid organ transplantation reduces T cell-mediated immune control of Epstein-Barr Virus (EBV), which may then drive development of post-transplant lymphoproliferative disease. Serology plays a key role in determination of risk of outgrowth of such lesions following transplantation. The study compared the VIDAS(®) (bioMérieux) and LIAISON(®) (DiaSorin) enzyme immunoassays (EIAs) and immunofluorescence assays (IFA; MBL-Bion) in the kidney transplantation setting. Sera from 100 live kidney donors [51 males; age range 20-82 years (mean 51.2 years)] and 100 cadaveric kidney recipients [70 males; age range 17-77 years (mean 51.0 years)] were tested. Overall proportional agreement ranged from 96% to 100% for VIDAS(®) and LIAISON(®). Sensitivity ranged from 91% to 100% and 92% to 100% for VIDAS(®)/IFA and LIAISON(®)/IFA, respectively. The VIDAS(®) and LIAISON(®) approaches gave similar results. Such automated random access EIAs are well suited to busy clinical virology laboratories and rapid determination of donor and recipient EBV serostatus prior to transplantation.

Copyright © 2014 Elsevier B.V. All rights reserved.

KEYWORDS:

Enzyme immunosorbent assay; Epstein–Barr Virus; Immunofluorescent assay; Kidney transplantation; Post-transplant lymphoproliferative disease; Serostatus

PMID:
24704349
[PubMed - indexed for MEDLINE]
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