Response to exogenous cholecystokinin of six human gastrointestinal cancers xenografted in nude mice

Am J Surg. 1989 Apr;157(4):386-94. doi: 10.1016/0002-9610(89)90582-5.

Abstract

Gastrointestinal hormones regulate growth of cancers as well as normal tissues. We investigated whether long-term cholecystokinin (CCK) administration might affect growth or metabolism of human tumors xenografted in nude mice. In each experiment, approximately 20 nude mice bearing subcutaneous xenografts of the particular cancer line being studied were used. Half received CCK and half received saline solution intraperitoneally twice daily for 14 days. Tumor volume and body weight were measured every 3 days. If the tumors produced marker substances, these were measured in nude mouse serum and also in the xenografts. Tumor growth was significantly retarded by CCK in two of the six cancers studied. In each case, DNA, RNA, and protein reflected tumor volumes. In one of these tumors (SLU 077), serum carcinoembryonic antigen (CEA) levels paralleled the tumor volumes. In another tumor (SLU 132), serum CEA levels and tumor immunolabeling for CEA and pancreatic oncofetal antigen increased in response to CCK administration, whereas tumor volumes did not. These findings suggest that exogenous highdose CCK altered the growth and metabolism in two of six human cancers studied.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Neoplasm / analysis
  • Biliary Tract Neoplasms / immunology
  • Biliary Tract Neoplasms / pathology
  • Biomarkers, Tumor / analysis
  • Carcinoembryonic Antigen / analysis
  • Cell Line
  • Cholecystokinin / pharmacology*
  • Gastrointestinal Neoplasms / immunology
  • Gastrointestinal Neoplasms / pathology*
  • Humans
  • Liver Neoplasms / immunology
  • Liver Neoplasms / pathology
  • Male
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Pancreatic Neoplasms / immunology
  • Pancreatic Neoplasms / pathology
  • Stomach Neoplasms / immunology
  • Stomach Neoplasms / pathology
  • alpha-Fetoproteins / analysis

Substances

  • Antigens, Neoplasm
  • Biomarkers, Tumor
  • Carcinoembryonic Antigen
  • alpha-Fetoproteins
  • oncofetal antigens
  • Cholecystokinin