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PLoS One. 2014 Mar 21;9(3):e92457. doi: 10.1371/journal.pone.0092457. eCollection 2014.

The role of HuR in the post-transcriptional regulation of interleukin-3 in T cells.

Author information

  • 1University of Puerto Rico-Río Piedras, Department of Biology, College of Natural Sciences, San Juan, Puerto Rico.
  • 2Department of Biochemistry, University of Puerto Rico-Medical Sciences, San Juan, Puerto Rico.
  • 3University of Puerto Rico-Río Piedras, Department of Biology, College of Natural Sciences, San Juan, Puerto Rico; Department of Biochemistry, University of Puerto Rico-Medical Sciences, San Juan, Puerto Rico; Molecular Sciences Research Building, San Juan, Puerto Rico.

Abstract

Human Interleukin-3 (IL-3) is a lymphokine member of a class of transiently expressed mRNAs harboring Adenosine/Uridine-Rich Elements (ARE) in their 3' untranslated regions (3'-UTRs). The regulatory effects of AREs are often mediated by specific ARE-binding proteins (ARE-BPs). In this report, we show that the human IL-3 3'-UTR plays a post-transcriptional regulation role in two human transformed cell lines. More specifically, we demonstrate that the hIL-3 3'-UTR represses the translation of a luciferase reporter both in HeLa and Jurkat T-cells. These results also revealed that the hIL-3 3'-UTR-mediated translational repression is exerted by an 83 nt region comprised mainly by AREs and some non-ARE sequences. Moreover, electrophoretic mobility shift assays (EMSAs) and UV-crosslinking analysis show that this hIL-3 ARE-rich region recruits five specific protein complexes, including the ARE-BPs HuR and TIA-1. HuR binding to this ARE-rich region appears to be spatially modulated during T-cell activation. Together, these results suggest that HuR recognizes the ARE-rich region and plays a role in the IL-3 3'-UTR-mediated post-transcriptional control in T-cells.

PMID:
24658545
[PubMed - indexed for MEDLINE]
PMCID:
PMC3962401
Free PMC Article
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