Format

Send to:

Choose Destination
See comment in PubMed Commons below
PLoS One. 2014 Mar 21;9(3):e91679. doi: 10.1371/journal.pone.0091679. eCollection 2014.

Concurrent measurement of dynamic changes in viral load, serum enzymes, T cell subsets, and cytokines in patients with severe fever with thrombocytopenia syndrome.

Author information

  • 1Department of Infectious Diseases, The First Affiliated Hospital, Nanjing Medical University, Nanjing, China; China-US Vaccine Research Center, The First Affiliated Hospital, Nanjing Medical University, Nanjing, China.
  • 2Department of Infectious Diseases, The First Affiliated Hospital, Nanjing Medical University, Nanjing, China.
  • 3China-US Vaccine Research Center, The First Affiliated Hospital, Nanjing Medical University, Nanjing, China; Laboratory of Nucleic Acid Vaccines, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America.

Abstract

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infection caused by a novel Bunyavirus. Analysis on the dynamic changes of clinical, laboratory, and immunological abnormalities associated with SFTS in a concurrent study is lacking. Thirty-three SFTS patients were admitted to Jiangsu People's Hospital, Nanjing, China, and diagnosis was made based on the clinical symptoms and positive viral RNA detected by RT-PCR. Four patients deceased and twenty-nine survived. Blood samples were collected every other day between Day 5 and Day 15 from the onset of fever. Samples from healthy volunteers were used as normal controls. Peak viral RNA load, serum enzymes, IL-6, and IL-10 were significantly higher in deceased patients compared to survivors. Viral load, serum enzymes, and cytokines declined in survivors within 2 weeks from onset of fever. CD69+ T cells were elevated early after infection while HLA-DR+ and CTLA4+ T cells were elevated during the recovery phase of those who survived. High level SFTSV viral load was concurrently observed with reduced PLT, elevated serum enzymes, elevated pro-inflammatory and anti-inflammatory cytokines, and activation of CD69+ T cells. The degree and pattern of changes in these parameters may indicate the clinical outcome in SFTSV-infected patients.

PMID:
24658451
[PubMed - indexed for MEDLINE]
PMCID:
PMC3962368
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk