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Biochem Biophys Res Commun. 2014 Apr 18;446(4):983-9. doi: 10.1016/j.bbrc.2014.03.051. Epub 2014 Mar 20.

Low immunogenicity of allogeneic human umbilical cord blood-derived mesenchymal stem cells in vitro and in vivo.

Author information

  • 1Biomedical Research Institute, MEDIPOST Co., Ltd, Seoul 137-874, Republic of Korea.
  • 2Research Institute for Future Medicine Stem Cell & Regenerative Medicine Center, Samsung Medical Center, Seoul 137-710, Republic of Korea.
  • 3Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul 139-709, Republic of Korea.
  • 4Biomedical Research Institute, MEDIPOST Co., Ltd, Seoul 137-874, Republic of Korea. Electronic address: jhb@medi-post.co.kr.

Abstract

Evaluation of the immunogenicity of human mesenchymal stem cells (MSCs) in an allogeneic setting during therapy has been hampered by lack of suitable models due to technical and ethical limitations. Here, we show that allogeneic human umbilical cord blood derived-MSCs (hUCB-MSCs) maintained low immunogenicity even after immune challenge in vitro. To confirm these properties in vivo, a humanized mouse model was established by injecting isolated hUCB-derived CD34+ cells intravenously into immunocompromised NOD/SCID IL2γnull (NSG) mice. After repeated intravenous injection of human peripheral blood mononuclear cells (hPBMCs) or MRC5 cells into these mice, immunological alterations including T cell proliferation and increased IFN-γ, TNF-α, and human IgG levels, were observed. In contrast, hUCB-MSC injection did not elicit these responses. While lymphocyte infiltration in the lung and small intestine and reduced survival rates were observed after hPBMC or MRC5 transplantation, no adverse events were observed following hUCB-MSC introduction. In conclusion, our data suggest that allogeneic hUCB-MSCs have low immunogenicity in vitro and in vivo, and are therefore "immunologically safe" for use in allogeneic clinical applications.

Copyright © 2014 Elsevier Inc. All rights reserved.

KEYWORDS:

Allogeneic; Human umbilical cord blood derived-mesenchymal stem cell (hUCB-MSC); Humanized NSG mouse; Low immunogenicity

[PubMed - indexed for MEDLINE]
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