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J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Apr 15;957:68-76. doi: 10.1016/j.jchromb.2014.03.001. Epub 2014 Mar 12.

Characterization of in vitro metabolites of JWH-018, JWH-073 and their 4-methyl derivatives, markers of the abuse of these synthetic cannabinoids.

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  • 1Dipartimento di Scienze Farmaceutiche, Università degli Studi di Milano, via Mangiagalli 25, I-20133 Milano, Italy.
  • 2Dipartimento di Scienze Farmaceutiche, Università degli Studi di Milano, via Mangiagalli 25, I-20133 Milano, Italy. Electronic address: gabriella.roda@unimi.it.

Abstract

In vitro incubation with human liver microsomes of JWH-018, JWH-073, JWH-122 and for the first time 1-butyl-3-(1-(4-methyl)naphthoyl)indole (the 4-methylnaphthoyl analogue of JWH-073) was investigated to identify the principal metabolites of alkylindole synthetic cannabinoids, thus helping the discovering of synthetic cannabinoids abusers. The results obtained showed that the most abundant metabolites were mono-hydroxylated derivatives either on the alkyl chain (ω or ω-1 position) or on the indole (presumably in position 5 or 6) and naphthalene moieties. Moreover the extraction conditions of these derivatives from biological fluids, mainly plasma and urine spiked with commercially available metabolite standards, and the incubation procedure were investigated to obtain a fast, reliable and suitable extraction protocol to detect either the parent drugs or their metabolites by means of GC/MS.

Copyright © 2014 Elsevier B.V. All rights reserved.

KEYWORDS:

Alkylindole metabolites; GC/FID; GC/MS; JWH-018; JWH-073; JWH-122 ;1-Butyl-3-(1-(4-methyl)naphthoyl)indole

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