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Mater Sci Eng C Mater Biol Appl. 2014 May 1;38:107-18. doi: 10.1016/j.msec.2014.01.045. Epub 2014 Feb 6.

In vitro study of manganese-doped bioactive glasses for bone regeneration.

Author information

  • 1Applied Science and Technology Department, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Turin, Italy. Electronic address: marta.miola@polito.it.
  • 2Applied Science and Technology Department, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Turin, Italy.
  • 3Department of Clinical and Biological Sciences, University of Turin, Via Zuretti 29, 10126 Turin, Italy.
  • 4Department of Public Health and Pediatric Sciences, Piazza Polonia, 94, 10126 Torino, Italy.
  • 5Department of Oncology, University of Turin, Via Santena 5/bis, 10126 Turin, Italy.
  • 6Department of Clinical and Biological Sciences, University of Turin, Corso Raffaello 30, 10125 Turin, Italy.

Abstract

A glass belonging to the system SiO2-P2O5-CaO-MgO-Na2O-K2O was modified by introducing two different amounts of manganese oxide (MnO). Mn-doped glasses were prepared by melt and quenching technique and characterized by means of X-ray diffraction (XRD), scanning electron microscopy (SEM) observation and energy dispersion spectrometry (EDS) analysis. In vitro bioactivity test in simulated body fluid (SBF) showed a slight decrease in the reactivity kinetics of Mn-doped glasses compared to the glass used as control; however the glasses maintained a good degree of bioactivity. Mn-leaching test in SBF and minimum essential medium (MEM) revealed fluctuating trends probably due to a re-precipitation of Mn compounds during the bioactivity process. Cellular tests showed that all the Mn-doped glasses, up to a concentration of 50 μg/cm(2) (μg of glass powders/cm(2) of cell monolayer), did not produce cytotoxic effects on human MG-63 osteoblasts cultured for up to 5 days. Finally, biocompatibility tests demonstrated a good osteoblast proliferation and spreading on Mn-doped glasses and most of all that the Mn-doping can promote the expression of alkaline phosphatase (ALP) and some bone morphogenetic proteins (BMPs).

Copyright © 2014 Elsevier B.V. All rights reserved.

KEYWORDS:

Bioactivity; Cytotoxicity; Gene expression; Genetic design; Mn-doped glasses

PMID:
24656359
[PubMed - indexed for MEDLINE]
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