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Hum Pathol. 2014 Apr;45(4):826-33. doi: 10.1016/j.humpath.2013.10.035. Epub 2013 Dec 18.

Human papillomavirus detection and p16INK4a expression in cervical lesions: a comparative study.

Author information

  • 1Department of Obstetrics and Gynecology, Hospital Universitari del Mar, 08003 Barcelona, Catalonia, Spain.
  • 2Department of Pathology, Hospital Universitari del Mar, 08003 Barcelona, Catalonia, Spain; School of Medicine, Hospital Universitari del Mar, Universitat Autònoma de Barcelona, 08003 Barcelona, Catalonia, Spain. Electronic address: 86780@parcdesalutmar.cat.
  • 3Department of Obstetrics and Gynecology, Hospital Universitari del Mar, 08003 Barcelona, Catalonia, Spain; School of Medicine, Hospital Universitari del Mar, Universitat Autònoma de Barcelona, 08003 Barcelona, Catalonia, Spain.
  • 4Department of Pathology, Hospital Universitari del Mar, 08003 Barcelona, Catalonia, Spain.
  • 5Department of Pathology, Hospital Germans Trias i Pujol, Barcelona, Catalonia, Spain.

Abstract

p16(INK4a) expression in dysplastic cervical lesions is related to high-risk human papillomavirus (HR-HPV) infection. The immunohistochemical expression of this protein in these lesions allows an increase in diagnostic reproducibility in biopsies and the introduction of prognostic factors in low-grade lesions. Here, we studied the immunohistochemical expression of p16 in 86 dysplastic cervical lesions, 54 cervical intraepithelial neoplasms-grade 1 (CIN-I), 23 CIN-II, and 9 CIN-III. In addition, we performed HPV detection and genotyping. We detected HR-HPV in 19/54 CIN-I, 21/23 CIN-II and 9/9 CIN-III cases. p16(INK4a) immunoreactivity was observed in 7/19 CIN-I HR-HPV-positive, 17/21 CIN-II HR-HPV-positive and all CIN-III cases. Immunoreactivity for p16(INK4a) was found in 7/54 CIN-I and in 17/23 CIN-II cases. In the follow-up, we detected 3 p16-positive high-grade squamous epithelial lesions (CIN-II and CIN-III) in the CIN-I/p16-negative group and 5 p16-positive high-grade squamous epithelial lesions cases in the CIN-II/p16-negative group. We conclude that p16 negativity in CIN-I and CIN-II biopsies does not always imply regression of the lesion and that the diagnosis of CIN-II should not be based solely on p16 results.

Copyright © 2014 Elsevier Inc. All rights reserved.

KEYWORDS:

Dysplasia; HPV; Uterine cervix; p16(INK4a)

PMID:
24656093
[PubMed - indexed for MEDLINE]
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