[Universal diagnostic oligonucleotide microarray for subtyping of human and animal influenza A viruses]

Vopr Virusol. 2013 Sep-Oct;58(5):32-7.
[Article in Russian]

Abstract

The diagnostic oligonucleotide microarray for subtyping of human and animal influenza A viruses (IAVs) was developed. We proposed a simple method of the fluorescent labeling of genomic segments of all known IAVs subtypes, the composition of the hybridization buffer, as well as the software of the data processing. 48 IAVs strains of different subtypes were analyzed using our microarray. All of them were identified, while 45 of 48 strains were unambiguously subtyped.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Genome, Viral*
  • Humans
  • Influenza A virus / classification*
  • Influenza A virus / genetics
  • Influenza A virus / isolation & purification
  • Influenza, Human / diagnosis
  • Influenza, Human / virology
  • Lab-On-A-Chip Devices
  • Molecular Typing / methods*
  • Oligonucleotide Array Sequence Analysis / instrumentation*
  • Orthomyxoviridae Infections / diagnosis
  • Orthomyxoviridae Infections / virology*
  • RNA, Viral / classification*
  • RNA, Viral / genetics
  • Software*

Substances

  • RNA, Viral